Journal: Biomedical research (Tokyo, Japan)
Article Title: Green coffee bean extract rich in chlorogenic acids prevents muscle capillary regression via inhibiting oxidative stress and enhancing angiogenesis.
doi: 10.2220/biomedres.46.15
Figure Lengend Snippet: Fig. 5 Effects of 50% and 5% chlorogenic acid on VEGF, TSP-1, ANG1, ANG2 and Tie-2 protein expressions. Band im- ages are representative western blots (A), and histograms show the quantification of band densities. Mean levels of VEGF (B), TSP-1 (C), VEGF/TSP-1 ratio (D), ANG1 (E), ANG2 (F), ANG2/ANG1 ratio (G) and Tie-2 (H) in the soleus muscles of each group. Values were calculated as the fold changes relative to the control group. Values are means ± SEM (n = 6). Ab- breviations: CON, control; HU, hindlimb unloaded; HU+50%CGA, hindlimb unloaded plus 50% chlorogenic acid; HU+5%CGA, hindlimb unloaded plus 5% chlorogenic acid. The symbols *, † and ‡ indicate significant differences from the CON, HU and HU+50%CGA groups, respectively, at P < 0.05.
Article Snippet: PBST, 23302-1-AP; Proteintech, Chicago, IL, USA), goat anti-Angiopoietin 2 (ANG2) (diluted 1 : 200 in PBST, sc-7015; Santa Cruz), rabbit anti-Tie-2 (diluted 1 : 1000 in PBST, 19157-1-AP; Proteintech), rabbit anti-Nuclear factor erythroid-derived 2-related factor 2 (Nrf2) (1 : 2000 in PBST, 16396-1-AP; Proteintech), rabbit anti-Heme oxygenase-1 (HO-1) (diluted 1 : 1000 in PBST, 10701-1-AP; Proteintech), rabbit anti-SOD2 (diluted 1 : 1000 in PBST, CSBPA006154; Cusabio Technology LLC, Houston, USA) and rabbit anti-Caspase 3 (diluted 1 : 1000 in PBST, 19677-1-AP; Proteintech).
Techniques: Western Blot, Muscles, Control
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